Microfluidics deals with the precise control of fluids on the microscale. Often at the microscale, less reagents are used resulting in faster and less expensive laboratories. In this experiment, a fluidic channel is created by casting the PDMS on a master made with puffy paint. PDMS is cured by an organometallic crosslinking reaction to give an optically transparent polymer. Different colored isopropanol is dispensed into the channels to observe fluid mixing.
- Wear eye protection
- Chemical gloves required
Step 1. Trace a petri dish. Mark the location of the holes in the plexiglas square. Draw a Y-shape connecting the holes.
Step 2. Place a petri dish over the paper with the pattern. Dispense puffy paint along the pattern.
Step 3. Measuring the viscous PDMS liquid can be messy. Cover the work surface and the balance with aluminum foil. Wear gloves.
Add xxxx g of Sylgard polymer base to a plastic cup. Add xxxx g of curing agent using a disposable dropper. Thorough mixing of the PDMS components is essential for good curing.
Step 4. After the puffy paint has hardened, add the PDMS mixture.
Step 5. Evacuate to remove bubbles from the PDMS. (It will look like even more bubbles appear in vacuum but when air is reintroduced the bubbles will pop.) Alternatively waiting and gently blowing over the surface may also eliminate bubbles.
Step 6. Cure the PDMS at 60 degrees C for an hour.
Step 7. Remove the PDMS from the Petri dish.
Step 8. Make a plexiglas sandwich with a PDMS filling, being sure that the holes drilled through the plexiglas align with the ends of the channel.
Step 9. Add colored isopropanol to each channel. Do the solutions mix?
- Plastic Petri dish
- Plexiglass rectangle with holes and not with holes
- Puffy paint
- PDMS base and curing agent
- Binder clips
- Plastic eyedroppers
- Isopropyl alcohol with blue food coloring
- Isopropyl alcohol with red food coloring